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. 2015 May 1;6:398. doi: 10.3389/fmicb.2015.00398

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Copyright © 2015 Cernava, Aschenbrenner, Grube, Liebminger and Berg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A qPCR approach was used to determine gene copy numbers of E. coli K12 after exposition to strong VOCs antagonists. TCVA-exposed samples were treated with PMA and gene copy numbers of viable cells were quantified with Unibac-II primers. Values for the treated samples were obtained from respectively six qPCR runs and additional 12 qPCR runs for untreated controls. Statistical analysis with ANOVA and one-sided t-test confirmed a highly significant decrease of gene copy numbers of the treated samples compared to the control group (P < 0.001).