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. 2015 May 1;6:295. doi: 10.3389/fpls.2015.00295

FIGURE 1.

FIGURE 1

OsCLE48 is an auxin response gene encoding an A-type CLE peptide. (A) Expression of OsCLE48 in response to auxin. Ten-days-old rice seedlings were treated with 10 μM IAA for 4 h, RNA was isolated and RT-PCR was used to examine the expression of OsCLE48. Expression of OsACT2 was used as a control. (B) Phylogenetic analysis of OsCLE48 and some of the A-type Arabidopsis CLEs. The entire amino acid sequences were used for analysis, and the phylogenetic tree was generated on Phylogeny (www.phylogeny.fr) by using the “One Click” mode with default settings. Branch support values are indicated above the branches. The accession codes for the Arabidopsis CLE genes are: CLV3, At2g27250; CLE1, At1g73165; CLE2, At4g18510; CLE3, At1g06225; CLE4, At2g31081; CLE5, At2g31083; CLE6, At2g31085; CLE7, At2g31082; CLE11, At1g49005; CLE12, At1g68795; CLE13, At1g73965; CLE25, At3g28455; CLE26, At1g69970; CLE27, At3g25905; CLE40, At5g12990. (C) Sequence alignment of OsCLE48 peptide and closed related A-type Arabidopsis CLE peptides. Identical amino acids are shaded in black, and similar amino acids in gray. (D) Expression of OsCLE48 in various tissues and organs of rice. RNA was isolated from roots and shoots of 7-days-old seedlings, and leaves, stem and spikelet of mature plants. Quantitative RT-PCR was used to examine the expression of OsCLE48. Rice UBQ5 was used as reference gene, and expression of OsCLE48 in root was set as 1. Data represent mean ± SD of three replications.