Figure 3.

Effect of RvE1 on CXC chemokine production in murine corneas. Corneas of C57BL/6 mice were abraded with three parallel, superficial scratches, and RvE1 (2 μg in 2 μL) was added topically. After 2 hours, 2 μL LPS (20 μg) or 2.5 μL tobramycin-killed P. aeruginosa or S. aureus (1 × 10⁷) was added topically, and 3 hours later, corneas were dissected and homogenized, and CXCL1 was measured by ELISA. This experiment is representative of two repeat studies with three mice per group. RvE1-treated corneas reduced chemokine production significantly. The overall P value by one-way ANOVA is < 0.0001 for all cytokines. *P < 0.05 derived from Tukey post hoc analyses. Data are shown as median and range.