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. 2015 Apr 30;56(4):2684–2695. doi: 10.1167/iovs.14-16190

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Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc.

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Analysis of retinal hcy levels and morphology in Mthfr^+/+ and Mthfr^+/− mice. Retinal hcy levels were quantified by HPLC and IHC analysis. (A) TheHcy standard curve used for calculation of retinal hcy levels. (B) Graphical representation of retinal hcy levels in Mthfr^+/+ and Mthfr^+/− mice (24 weeks) expressed as pmol/μg protein. ***Significantly different from wildtype mice, P < 0.001. (C, D) Retinal cryosections from Mthfr^+/+ and Mthfr^+/− mice (24 weeks) were subjected to immunodetection of hcy; Alexa Fluor 555-conjugated antibody (red) was used to detect positive signals. (E) The immunopositive signals were quantified using Metamorph software. **Significantly different from wild type mice, P < 0.01. (F) Representative hematoxylin and eosin–stained sections of retinas harvested from Mthfr^+/+ and (G, H) Mthfr^+/− mice (24 weeks). Areas of cellular dropout observed in retinas from Mthfr^+/− mice are noted by the black arrow (H). Calibration bar: 50 μM. ipl, IPL; opl, OPL; is, IS; os, outer segment.