Fig. 5.
Pharmacology of fast depolarization and pharmacological separation of multiple responses. A: example and summary of the pharmacology of the fast depolarization in a ChAT-ChR2(Ai32) mouse. Example: sequential addition of mec/atr, 100 μM mecamylamine and 1 μM atropine; GBZ/CGP, 1 μM gabazine and 3 μM CGP 52432; NBQX/CPP, 10 μM NBQX and 10 μM CPP; 2-ms illumination. Neuron at rest. Summary: means ± SE, normalized to predrug amplitude. mec/atr, GBZ/CGP, and NBQX/CPP, 5, 3, and 3 neurons, respectively. Antagonists were added sequentially in each recording. B: example of the effect of sequential application of drugs to a neuron from a ChAT-ChR2(Ai32) mouse with a combination of all 4 responses. Bottom: voltage responses at resting membrane potential (Vm). Top: voltage responses during depolarizing somatic current injection.