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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Methods Mol Biol. 2014;1156:53–66. doi: 10.1007/978-1-4939-0685-7_4

Fig. 1.

Fig. 1

A schematic overview of the in-gel digestion procedure. Protein sample is separated by 1D SDS-PAGE, and the lane is cut into bands for parallel processing. Reduction and alkylation are performed prior to enzymatic digestion of proteins. Peptides are extracted for LC-MS/MS analysis followed by protein sequence database searches