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. Author manuscript; available in PMC: 2015 May 10.
Published in final edited form as: Physiol Behav. 2014 Apr 13;130:135–144. doi: 10.1016/j.physbeh.2014.04.011

Table 2.

Analysis of proteins involved in neuroprotection and inflammation.

Molecules involved in Memory consolidation Hippocampus Amygdala Frontal Cortex
Con Ex SD SD + Ex Con Ex SD SD + Ex Con Ex SD SD + Ex
BDNF/β-actin 1.25+0.03 1.37±0.01 0.55±0.04* 1.07+0.01# 1.31±0.02 1.40±0.04 0.91±0.02 1.10±0.07 1.28±0.01 1.31±0.02 1.22±0.02 0.97±0.07
p-CREB/t-CREB 0.48±0.05 0.43±0.03 0.22±0.01* 0.38±0.03# 0.21±0.03 0.33±0.01 0.16±0.01 0.14±0.02 0.34±0.03 0.40±0.05 0.31±.05 0.30±.01
CAMKIV/β-actin 0.55±0.02 0.53±0.02 0.20±0.03* 0.41±0.02# 0.42±0.02 0.43±0.04* 0.36±0.07 0.34±0.02 0.51±0.06 0.60±0.04 0.53±.03 0.50±.02
Inflammatory Markers
p-ERK[1/2)/t-ERK(1/2) 0.23+0.04 0.20±0.03 0.57±0.04* 0.38±0.01# 0.20±0.02 0.22+0.03 0.60±0.03* 0.41±0.01# 0.33±0.02 0.30±0.03 0.37±0.02 0.4±0.01
IL-6/β-actin 0.44+0.01 0.42±0.02 0.80±0.01* 0.58±0.04# 0.50±0.01 0.42±0.02 0.60±0.01 0.58±0.04 0.54±0.02 0.42±0.02 0.90±0.03* 0.68±0.03#

Protein levels of BDNF/β-Actin, p-CREB/t-CREB, CAMKIV/β-Actin, p-ERK1/2/t-ERK1/2 and IL-6/β-Actin were determined by Western blotting in the hippocampus, amygdala and the frontal cortex. Protein ratios were obtained by normalizing to loading control/total protein as indicated. Group 1: control (sedentary, CON), group 2: exercised (EX), group 3: social defeat (SD); group 4: social defeat and exercise (SD+EX).

*

significantly different from control (sedentary and exercise alone) rats (p<0.05),

#

significantly different from SD rats (p<0.05) using one way ANOVA analysis, n = 4–6 rats/group.