Table 1. Summary of candidate gene validation experiments.
| RNAi Driver | |||||
|---|---|---|---|---|---|
| Candidate Gene | GWAS Association | Exelixis (T5/T6) | tub-GAL4 (T5/T6) | pnr-GAL4 (T5/T6) | ubi-GAL4 (T5/T6) |
| CG33298 | T6 | NS/NS | NS/NS | NS/NS | - |
| Fish-lips (Fili) | T6 | ↑/↑ | lethal (♀ only) | lethal (♀ only) | - |
| Vesicular monoamine transporter (Vmat) | T6 | - | lethal | NS/NS | lethal |
| multiple wing hairs (mwh) | T6 | NS/NS | NS/NS | NS/NS | - |
| Kinesin-like protein at 61F (Klp61F) | T6 | NS/NS | lethal | NS/↑ | lethal |
| CG9134 | T6 | ↑/↑ | ↓/↓ | NS/NS | - |
| CG7852 | T6, T5-T6 | ↑/↓ | lethal | NS/↓ | lethal |
| CG1887 | T6 | - | lethal | NS/NS | ↓/NS |
| klarsicht (klar) | T6 | NS/↓ | lethal | NS/↑ | ↑/↑ |
| Glucose transporter 1 (Glut1) | T6 | ↑/↑ | - | - | - |
| Exchange factor for Arf 6 (Efa6) | T6 | ↑/↑ | ↑/↑ | NS/↑ | - |
| buttonless (btn) | T6 | - | ↓/↓ | NS/↓ | - |
| roughoid (ru) | T5 | - | lethal | ↓/↓ | lethal |
| CG10625 | T5 | NS/↓ | ↓/↓ | NS/NS | - |
| sinuous (sinu) | T5 | - | lethal | ↓/↓ | lethal |
| Sucb (Sucb) | T5 | NS/↓ | - | - | - |
| division abnormally delayed (dally) | T5 | NS/↓ | NS/NS | NS/NS | - |
| CG32052 | T5 | - | NS/NS | NS/NS | - |
| Nedd2-like caspase (Nc) | T5 | - | lethal | NS/NS | lethal |
| Ceramide kinase (Cerk) | T5 | - | NS/NS | NS/NS | - |
| krotzkopf verkehrt (kkv) | T5 | ↓/↓ | lethal | lethal | lethal |
| CG15803 | T5 | - | NS/NS | NS/NS | - |
| locomotion defects (loco) | T5 | NS/↓ | ↑/↑ | NS/NS | - |
| TweedleC (TwdlC) | T5 | - | NS/NS | NS/NS | |
| kayak (kay) | T5 | NS/↓ | lethal | NS/NS | lethal |
| CG1340 | T5 | - | NS/NS | NS/NS | - |
| CG42594 | T5 | - | NS/NS | NS/NS | - |
| CG42340 | T5 | - | lethal | NS/NS | lethal |
NS = not significant,"–" = line not available or RNAi cross not tested. ↑ = increased pigmentation. ↓ = decreased pigmentation.