Fig 4. Activation of tumorigenic pathways in hepatocytes of HBV transgenic mice.

Western blot analysis of total protein lysates from the liver of 12-, 26-, and 52-week-old mice was performed using (A) anti-Jun, (B) anti-phospho-SAPK/JNK, (C), anti-phospho-c-Jun and (D) anti-phospho-STAT3 antibodies. Samples were loaded as described in the legend of Fig 1 except (A) panel 26 weeks, where the loading was as follows: 1—female BALB/c; 2,3—female HBVTg/c; 4—male BALB/c; 5, 6—male HBVTg/c; 7—female C57BL/6; 8—female HBVTg/6; 9—male C57BL/6; 10—female HBVTg/6, 11, 12—male HBVTg/6 mice. Equal protein loading was confirmed with anti-β-actin (A, B and C) and anti-STAT3 (D) antibodies.