FIGURE 5.
CD28 activation increases BLIMP-1 and BMCA expression in BM PC. (A) BM and splenic PC were purified from BLIMP-1gfp reporter mice and cultured ± control Ig-coated beads or αCD28 mAb-coated beads for 16 h, and analyzed for BLIMP-1-GFP expression by flow cytometry. Results representative of three independent experiments. Quantification of three independent experiments shown on right and represents the aggregate mean ± SD of these experiments. (B) BM and splenic PC purified from BLIMP-1gfp mice were cultured with control Ig-coated beads or αCD28 mAb-coated beads as above, and stained for BCMA and analyzed by flow cytometry. Data representative of two independent experiments. **p < 0.01.