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. 2015 Apr 13;194(10):5014–5021. doi: 10.4049/jimmunol.1402991

FIGURE 5.

FIGURE 5.

Histamine promotes the accumulation of intratumoral DCs and reduces EL-4 lymphoma growth in vivo in a NADPH oxidase/NOX2–dependent manner. (A) Growth of EL-4 lymphoma cells implanted s.c. into C57BL/6 mice treated with histamine (his; red bars) or NaCl (open bars). (B and E) Percentage of CD11c+IaIe+ splenic and intratumoral DCs after 13 d of tumor growth. (C) FACS plots of intratumoral DCs in a representative control (Ctl) mouse and corresponding results in a histamine-treated mouse (+his). Live (DAPI−) hematopoietic (CD45+) cells are displayed. (D) Tumor growth of EL-4 lymphoma in NOX2−/− C57BL/6 mice during treatment with histamine (red bars) or NaCl (open bars). In (A), (B), (D), and (E), the boxes represent the medians and quartiles, and the error bars show the minimum to maximum values. (F) Correlation between the percentage of CD11c+IaIe+ intratumoral DCs and tumor size after 13 d of growth for WT and NOX2−/− C57BL/6 mice. (G) Correlation between the percentage of CD3+CD8+ intratumoral T cells and tumor size after 13 d of growth for WT and NOX2−/− C57BL/6 mice. (H) Correlation between the percentage of CD11c+IaIe+ intratumoral DCs and the percentage of CD3+CD8+ intratumoral T cells. **p < 0.01, ***p < 0.001 Student t test.