Table II. NK cell responses to vaccine Ags by HCMV status after adjusting for sex and age.
| Adjusted for Sex and Age |
|||
|---|---|---|---|
| Stimulus | Parameter (Total NK Cells) | Effect (95% CI)a | pb |
| Pertussis | CD25+ | −4.4 (−8.3, −0.5) | 0.014 |
| CD25+IFN-γ+ | −0.5 (−1.2, 0.3) | 0.125 | |
| CD107a+ | −1.5 (−3.4, 0.5) | 0.071 | |
| Pertussis + LCC | CD25+ | −8.5 (−13.7, −3.4) | 0.001 |
| CD25+IFN-γ+ | −1.5 (−2.8, −0.1) | 0.020 | |
| CD107a+ | −2.9 (−5.5, −0.3) | 0.016 | |
| H1N1 | CD25+ | −5.4 (−9.5, −1.3) | 0.005 |
| CD25+IFN-γ+ | −0.4 (−1.1, 0.4) | 0.158 | |
| CD107a+ | −1.8 (−3.9, 0.3) | 0.049 | |
| H1N1 + LCC | CD25+ | −12.2 (−22.6, −1.8) | 0.011 |
| CD25+IFN-γ+ | −5.1 (−10.4, 0.1) | 0.027 | |
| CD107a+ | −5.1 (−8.9, −1.5) | 0.004 | |
| HCC | CD25+ | −11.3 (−16.7, −6.0) | <0.0001 |
| CD25+IFN-γ+ | −6.5 (−11.4, −1.7) | 0.005 | |
| CD107a+ | −2.1 (−3.5, −0.6) | 0.004 | |
A regression analysis was performed in STATA to adjust for sex and age when comparing NK cell responses to pertussis (−/+ LCC), H1N1 (−/+ LCC), and HCC between HCMV− and HCMV+ donors. The response was quantified by the percentage of total NK cells expressing CD25, CD25/IFN-γ (CD25+IFN-γ+), and CD107a.
a
Effect (coefficient), with 95% confidence interval (CI), represents the change in the mean percentage of NK cells responding in HCMV+ donors as compared with HCMV− donors.
b
The p value refers to the significance of the difference in response between HCMV− and HCMV+ donors after adjusting for sex and age. The p values < 0.05 are underlined.