Fig 3. Peptide loss during 1 h incubation of 220 μ L solutions in sample containers.

The percentage of recovered peptide was measured as a function of the peptide concentration for mastoparan X (A), melittin (B), and magainin 2 (C) solutions in borosilicate glass vials, polypropylene tubes, or Protein LoBind tubes. In all panels, the data are the average of two separate experiments. The error bars show the standard deviations. The error bars are not shown if they are smaller than the symbols. The data demonstrate that all three peptides tend to adsorb to the walls of the borosilicate glass vials and polypropylene tubes; at low peptide concentrations, only 10–20% of the expected peptide contents were recovered in these containers. In contrast, peptides do not absorb to Protein LoBind tubes to the same extent.