Figure 7.

Internal limiting membrane immunohistochemistry and transmission electron microscopy. (A) In human retinas treated with the DDMS, laminin A antibodies (green) identified the ILM. Cell nuclei were stained with DAPI (blue). ONL, outer nuclear layer. (B) Transmission electron microscopy image of untreated retinas showed Müller glial (MG) cell end feet processes at the edge of the ILM and filopodial insertions deeper within the ILM. (C) Treatment of retinas with medium pressure from the Tano resulted in removal of most of the ILM and the deepest Müller filopodial insertions, but preserved a thin layer of ILM over the MG end feet.