Table 3. Kinetics parameters of wild-type GOOX and CBM fusions on cellotetraose.
| Enzymes | k cat (min-1) | K m (mM) | k cat/K m a (min-1. mM-1) |
|---|---|---|---|
| Wild-type GOOX | 250 ± 20 a | 0.07 ± 0.02 | 3,400 ± 700 |
| CtCBM3-GOOX | 300 ± 20* | 0.10 ± 0.02 | 3,100 ± 700 |
| CtCBM11-GOOX | 310 ± 20* | 0.10 ± 0.02 | 3,100 ± 700 |
| CtCBM44-GOOX | 380 ± 30* | 0.12 ± 0.03 | 3,100 ± 700 |
| GOOX-CtCBM3 | 150 ± 10 | 0.05 ± 0.01 | 2,900 ± 600 |
| GOOX-CtCBM11 | 360 ± 30* | 0.11 ± 0.02 | 3,200 ± 700 |
| GOOX-CtCBM44 | 290 ± 20 | 0.09 ± 0.02 | 3,300 ± 700 |
Errors indicate standard deviations; n = 3.
a Standard deviations (SD) for k cat/K m values were calculated using following formula: SD k cat/K m = k cat/K m × [SQRT((SD(K m)/K m)2 + (SD(k cat)/k cat)2)].
Asterisk indicates statistically significant increases compared to wild-type GOOX as determined using a two-tailed t-test for two samples with unequal variance (p < 0.05).