Table 5. The half-life of wild-type GOOX and CBM fusions at 45°C.
Enzymes | Half life (min) |
---|---|
Wild-type GOOX | 119 ± 4 |
CtCBM3-GOOX | 132 ± 2* |
CtCBM11-GOOX | 132 ± 2* |
CtCBM44-GOOX | 127 ± 4 |
GOOX-CtCBM3 | 104 ± 9 |
GOOX-CtCBM11 | 111 ± 10 |
GOOX-CtCBM44 | 170 ± 20* |
Values were obtained by plotting the log (% residual activity) versus incubation time (min). Errors indicate standard deviations; n = 3. Asterisk indicates statistically significant improvements compared to wild-type GOOX as determined using a two-tailed t-test for two samples with unequal variance (p < 0.05)