Fig 7. CD24 supports initiation and progression of mesotheliomas.
(A) Mock-transfected and CD24- Ist-Mes-2 cells (106 per animal) were grafted subcutaneously into NOD/SCID mice and tumour formation followed using USI. (B) CD24- and mock-transfected Ist-Mes-2 cell-derived tumours were evaluated for CD24, CD47, EpCAM and Oct3/4 by western blotting using anti-actin IgG as a loading control, with panel C documenting densitometric evaluation of the blots. (D) A representative image of a mouse with CD24- cell-derived tumour and parental cell-derived tumour is shown. (E) Representative USI images of a tumour derived from mock-transfected and CD24- cells acquired on different days are shown, with the yellow arrows indicating the position of the tumours. Data in panel A are mean values ±S.E.M., and are derived from four animals. The symbol ‘*’ denotes statistically significant differences with p<0.05. Images in panel B are representative of two independent experiments with the densitometric evaluation showing average data with differences less than 10%.