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. 2015 May 1;10(5):e0119549. doi: 10.1371/journal.pone.0119549

Fig 8. Effect of CD24 knock-down on mitochondrial function.

Fig 8

Parental and CD24- adherent and sphere Ist-Mes-2, non-permeabilised cells were evaluated for routine respiration using the Oxygraph instrument (A). Panel B shows respiration related to the maximum respiratory capacity of the cells (ETC). The symbols in panel A stand for: R, routine, L, leak, E, ETC, netR, R-L, ROX, residual respiration. Parental, mock-transfected, CD24- adherent and sphere Ist-Mes-2 cells were evaluated for ΔΨm using TMRM (C), superoxide generation using MitoSOX (D), lactate production using a commercial kit (E), citrate synthase (CS) activity (F), relative SDH (G) and SQR activity (H), glucose uptake (I) and ATP level (J). Panel K documents the level of PCG1αmRNA in parental, mock-transfected and CD24- adherent and sphere Ist-Mes-2 cells. Data shown are mean values ±S.D., and are derived from three individual experiments. The symbol ‘*’ denotes statistically significant differences with p<0.05.