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. 2015 May 1;10(5):e0125022. doi: 10.1371/journal.pone.0125022

Fig 4. Blocking of G-protein signalling reduces CFA and carrageenan-induced mechanical hyperalgesia.

Fig 4

(A) Mice were injected with 25 μl of AC inhibitor (SQ22536) before CFA injection or with Gi inhibitor (pertussis toxin [PTX]), PLCβ inhibitor (U73122), or Gβγ inhibitor (gallein) 1 day after CFA injection. Data are mean±SEM of total tested mice (n = 6–12 per group). #$*p<0.05, &&P<0.01, $$$###&&&***p<0.001 compared to no inhibitor-injected groups by one-way ANOVA with a post-hoc Bonferroni test. (B-C) Mice were injected with AC inhibitor (SQ22536, 1 mM), Gi inhibitor (PTX, 100 ng) or PLCβ inhibitor (U73122, 500 μM), before (0 h) or 4 h or 1 day after CFA (B) or carrageenan (C) injection. The threshold of paw withdrawal was measured at 90 min after the second injection. Data are mean±SEM of total tested mice (n = 6–8 per group). $$$###&&&***p<0.001 compared to CFA-injected or carrageenan-injected groups by two-way ANOVA with a post-hoc Bonferroni test.