Skip to main content
. 2015 May 1;10(5):e0125623. doi: 10.1371/journal.pone.0125623

Fig 2. IL17 is essential for development of EAP induced pain in C57BL/6 mice.

Fig 2

A. anti-IL17 blocking antibody or an IgG isotype control were given by i.p. injection one day prior to induction of EAP and one day prior to tactile allodynia testing every 7 days for 28 days. (i) Percentage response frequency curves demonstrating no increase in pain above baseline in anti-IL17 treated group compared to IgG control. (ii-iv) Response frequency to individual filaments for each mouse group. B. Flow cytometry analyses for T-cell markers in prostate and iliac lymph node tissues from these mice for (i, iv) (ii, v) CD4+ve IL17 +ve, (iii, vi) CD4+ve IFNγ and CD4+ve CD25+ve FoxP3+ve cells. Mean +/- SEM, gating strategy is outlined in S1 Fig Statistics were performed in Prism software using, Two-way ANOVA for response change analyses, and unpaired student’s T-tests for flow, averages of two separate experiments N = 4/5 mice per group displayed for both. * = p<0.05, **** = p<0.0001.