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. 2015 May 1;10(5):e0125623. doi: 10.1371/journal.pone.0125623

Fig 5. IL7 expression is increased in a prostate specific manner in EAP mice.

Fig 5

A (i-iv) QRTPCR of IL7 from murine splenic, bladder, prostate and iliac lymph tissues. B. Immuno-blotting and densitometry for IL7 using prostate tissue lysates. C. Immuno-histochemical staining for IL7, (i-ii) Isotype staining on EAP prostate (iii-iv) IL7 staining on control prostate and (v-vi) IL7 staining on EAP prostate, circle; epithelial, arrowhead; stromal cell staining. Immunoblot densitometry performed in ImageJ using area under the curve values followed by a T-test. Mean +/- SEM are shown, statistics performed in Prism using unpaired T-test, QRTPCR samples normalized to relevant controls with L19 as housekeeping gene and displayed as fold change, performed in technical duplicate and biological triplicate with N = 4/5 mice per group. * = p<0.05, ** = p<0.01.