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. 2015 May 1;10(5):e0125928. doi: 10.1371/journal.pone.0125928

Fig 3. Effect of HIV PIs on mRNA levels of the UPR genes in HNSCC.

Fig 3

HNSCC cells, SQ20B and FaDu, were cultured in complete medium and treated with different concentrations of L/R (0, 12.5, 25, and 50 μM) or TG (50 nM) for 24h. At the end of treatment, total RNAs were isolated and reverse transcribed. The relative mRNA levels of ATF-4, CHOP, spliced XBP-1 (XBP-1s) and unspliced XBP-1 (XBP-1u) were detected by real-time RT-PCR and normalized using GAPDH as an internal control as described under “Methods”. Values are mean ± S.E. of three independent experiments. Statistical significance relative to vehicle control, *P<0.05; **P<0.01; ***P<0.001. (A) SQ20B cells; (B) FaDu cells.