Fig 5. Effect of HIV PIs on apoptosis in SQ20B and FaDu cells.

Human HNSCC cells SQ20B and FaDu were cultured in complete medium and treated with HIV PIs L/R (25μM) or TG (50 nM) for 24h. At the end of treatment, cells were stained with Annexin V- PI and analyzed using the Cellometer Vision CBA system. FL1 represents Annexin V staining for apoptotic cells and FL2 represents PI staining for necrotic cells. Representative images of flow cytometry analysis and percentages of apoptotic and necrotic cells from three independent experiments are shown: (A) SQ20B; (B) FaDu. Statistical significance relative to vehicle control, *P<0.05.