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. 2015 May 1;10(5):e0123187. doi: 10.1371/journal.pone.0123187

Fig 3. p53 and PAI-1 are prominently linked to PCSE-induced type II AEC apoptosis.

Fig 3

(A) WT, p53- and PAI-1-deficient mice were exposed to ambient air (control) or PCS for 20 weeks. Lung section obtained from these mice were subjected to TUNEL staining and the bar graph represents percent apoptosis in these groups with error bars and significance *(p<0.005) (n = 5 mice/group). (B) Type II AECs isolated from WT, p53- and PAI-1-deficient mice as described in methods were subjected to TUNEL staining and the bar graph represents percent apoptosis in these groups with error bars and significance *(p<0.005) (n = 5 mice/group). (C) Type II AECs isolated from WT, p53- and PAI-1-deficient mice were subjected to flow cytometric analysis after staining with anti-annexin-v antibody and PI to assess apoptosis. NS = the differences are not statistically significant (n = 5 mice/group). (D) Type II AECs isolated from WT and p53- and PAI-1-deficient mice as described above were immunoblotted for SP-C and β-actin as a loading control.