Fig 5. (A) Map showing the Ad-vector harboring SP-B promoter plus chimeric luciferase cDNA having either p53-binding (Ad-PAI-1⁷⁰⁺) or corresponding control (Ad-PAI-1^70-) PAI-1 3’UTR sequence.

(B) Type II AECs isolated from WT mice were transduced with Ad-vector alone or Ad-PAI-1⁷⁰⁺ or Ad-PAI-1^70- in vitro. One day after transduction, these cells were either treated with PBS or 1.5% of CS extract (CSE, O.D. = 1.00 at 260 nm = 100%) for additional 24h. Conditioned media (CM) were tested for changes in PAI-1 and EAR1, and the cell lysates (CL) were immunoblotted for p53, luciferase and active caspase-3. (C) Total RNA obtained from WT AECs as described above was analyzed for EAR mRNA by RT-PCR. Experiments were repeated at least two times.