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. 2015 May 1;10(5):e0123808. doi: 10.1371/journal.pone.0123808

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© 2015 Jaiswal et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — The LP-BER was determined using an in vitro reconstituted assay system. Panel A shows the experimental protocol. The assay details are given in Materials and Methods. Panel B shows an autoradiogram of LP-BER, which is representative of three different experiments. Lane 1 shows ³²P-labeled 63-mer F-DNA and Lane 2 shows the 23-mer product after APE1 incision. Lane 3 shows Pol-β-mediated strand-displacement synthesis. Lane 4 shows Pol-β-mediated strand-displacement synthesis stimulated by Fen1 (Lane 4). Lane 5 shows the complete repair of the 63-mer F-DNA through the LP-BER pathway in the presence of APE1, Pol-β, Fen1 and DNA ligase I. Lanes 6–9, 10–13, 14–17, 18–21 and 22–25 show the effect of different concentrations of the Pol-β inhibitors NSC 661073, 666713, 666715, 666717 and 666719 on LP-BER activity.