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. 2015 May 1;10(5):e0123808. doi: 10.1371/journal.pone.0123808

Fig 3. NSC666715 and its analogs cause the accumulation of TMZ-induced AP sites in the HCT116 cell line.

Fig 3

Cells were pretreated for 2 h with 25 μM of the small molecule inhibitors NSC666715, NSC666717 and NSC666719, followed by treatment with 500 μM of TMZ for an additional 48 h. Genomic DNA was isolated and processed for AP site determination as described in Materials and Methods. Panel A shows the slot blot analysis of AP sites in HCT116 cells treated with 25 μM of NSC666715, NSC666717 and NSC666719 and 500 μM TMZ. Panel B shows the quantitative analysis of AP sites. Results are presented as mean ± SE of three different determinations. * = Significantly different than the untreated control group (P<0.05).