Table 1. Effect of PFTα on the cell cycle phases of HCT116 cells treated with TMZ and NSC666715 either alone or in combination.
| % change | ||||
|---|---|---|---|---|
| Treatment | G0/G1 | S | G2/M | sub-G1 (Apoptosis) |
| Control | 83 ± 4.4 | 10 ± 2.8 | 7 ± 1.9 | 3 ± 1.3 |
| 500 μM TMZ | 54 ± 1.6 § | 33 ± 1.2 § | 13 ± 1.3 | 6 ± 1.3 |
| 50 μM NSC666715 | 83 ± 1.4 | 11 ± 2.1 | 6 ± 0.7 | 2 ± 0.2 |
| 10 μM PFTα | 61 ± 5.9 § | 29 ± 3.6 § | 10 ± 2.3 | 5 ± 1.1 |
| 20 μM PFTα | 73 ±. 07 | 20 ± 1.1 § | 7 ± 0.6 | 5 ± 2.3 |
| 30 μM PFTα | 79 ± 2.7 | 13 ± 2.1 | 7 ± 0.8 | 3 ± 0.4 |
| 10 μM PFTα + 50 μM NSC666715 | 73 ± 1.3 | 19 ± 1.3 § | 8 ± 0.7 | 6 ± 2.1 |
| 20 μM PFTα + 50 μM NSC666715 | 68 ± 5.5 | 21 ± 2.8 | 11 ± 2.9 | 7 ± 1.8 |
| 10 μM PFTα + 500 μM TMZ | 67 ± 1.7 § | 24 ± 1.0 § | 9 ± 0.9 | 8 ± 3.5 |
| 20 μM PFTα + 500 μM TMZ | 69 ± 3.1 | 21 ± 1.8 § | 10 ± 1.5 | 8 ± 3.3 |
| 30 μM PFTα + 500 μM TMZ | 75 ± 5.8 | 18 ± 2.1 | 8 ± 3.8 | 7 ± 2.8 |
| 10 μM PFTα + 500 μM TMZ +50 μM NSC666715 | 69 ± 1.0 § | 21 ± 0.2 § | 10 ± 0.8 | 8 ± 1.9 |
| 20 μM PFTα + 500 μM TMZ +50 μM NSC666715 | 69 ± 5.5 | 20 ± 4.5 | 11 ± 0.5 | 10 ± 2.3 |
| 30 μM PFTα + 500 μM TMZ +50 μM NSC666715 | 69 ± 2.1 § | 21 ± 3.5 | 11 ± 1.5 | 9 ± 1.6 |
Cells were treated with different concentrations of drugs as indicated for 48 h. Cell cycle status and apoptosis were examined by FACS analysis. The percent distribution of cells in the G0/G1, S, G2/M and sub-G1 (apoptotic) phases of the cell cycle were established on the basis of the corresponding DNA content of the histograms. At least 10,000 cells per sample were considered in the gated regions for calculations. Data are presented as mean ± SE of three different estimations.
§ = Significantly different than the control (p <0.05).