Table 2. Alignment statistics for EBV-1 and EBV-2.

Alignment statistics vs. EBV-1
	B95.8	Jijoye	LCL1	LCL3	LCL9	LCL10
Total number of raw reads	462,896	454,186	389,274	335,880	564,928	542,650
Aligned reads	97.3%	89.9%	95.2%	96.1%	92.9%	92.7%
- Uniquely matched reads	97.1%	89.5%	94.9%	95.5%	92.5%	91.8%
- Number of uniquely aligned reads	449,472	406,496	369,421	320,765	522,558	498,153
Unaligned reads	2.7%	10.1%	4.8%	3.9%	7.1%	7.3%
Average read length	146	142	144	143	144	144
Total Aligned Throughput (Mb)	65.6	57.7	53.2	45.9	75.2	71.7
Average Coverage	382	336	310	267	438	417
Alignment statistics vs. EBV-2
Total number of reads	462,896	454,186	389,274	335,880	564,928	542,650
Aligned reads	91.9%	95.3%	93.1%	85.9%	87.7%	95.6%
- Uniquely matched reads	91.8%	94.8%	92.7%	85.5%	87.3%	94.6%
- Number of uniquely aligned reads	424,939	430,568	360,857	287,177	493,182	513,347
Unaligned reads	8.1%	4.7%	6.9%	14.1%	12.3%	4.4%
Average read length	146	142	144	144	144	143
Total Aligned Throughput (Mb)	62.0	61.1	52.0	41.4	71.0	73.4
Average Coverage	359	354	301	239	411	425

QC performance against EBV-1 showed a robust performance with high raw reads, high aligned reads, uniform average length read for each sample, and a normal average coverage for the samples. In QC performance against EBV-2 we also observed similar QC performance on alignment against EBV-2 with high raw reads, high aligned reads, uniform average length read for each sample, and a normal average coverage for the samples.