FIGURE 12.

BMP7-driven alterations in myofibroblast phenotype are Hyal2-dependent. Cells were grown to subconfluence and transfected with specific siRNA nucleotides targeting Hyal1 (A and C) and Hyal2 (B and D) for 24 h prior to the indicated cellular treatments according to experimental models before extraction with TRIzol reagent. RT-QPCR was used to assess Hyal1 (A), Hyal2 (B), and α-SMA (C and D) mRNA expression. Results are shown as the mean ± S.E. of three individual experiments. Statistical significance used the paired Student's t test, and p < 0.05 was considered as significant. E and F, cells were grown to subconfluence before transfection with specific siRNA nucleotides targeting Hyal1 or Hyal2 for 24 h prior to the indicated cellular treatments. Cells were subsequently fixed and stained for α-SMA (E) and HABP (f). Original magnification ×400 Images shown are representative of three individual experiments.