FIGURE 3.

BMP7 treatment alters cellular HA distribution resulting in internalization of HA in myofibroblasts. Cells were grown to confluence in 35-mm dishes; growth-arrested for 48 h, and incubated with the indicated cytokine treatments in the experimental models as described under “Experimental Procedures.” HA ELISA was used to analyze the concentrations of HA in conditioned cell culture media (A), cell-surface trypsinates (B), and intracellular lysates (C). Results are expressed as the mean ± S.E. of three individual experiments. Statistical analysis used the paired Student's t test with p < 0.05 considered as statistically significant. N/S, not significant.