FIGURE 5.

BMP7 alters HA-associated gene expression. Fibroblasts were grown to confluence before undergoing growth arrest in serum-free medium for 48 h. Cells were then treated under various cytokine conditions according to the experimental models described under “Experimental Procedures” before extraction with TRIzol reagent. RNAs were purified, reverse-transcribed and assessed for: HAS1 (A), HAS2 (B), HAS3 (C), CD44 (D), Hyal1 (E), and Hyal2 (F) mRNA expression using RT-QPCR. Results are shown as the mean ± S.E. of three individual experiments. Statistical analysis used the paired Student's t test with p < 0.05 considered as statistically significant, and comparison is made between control and all other treatment conditions.