FIGURE 8.

Distribution of Hyal1 and Hyal2 in endosomes and lysosomes in fibroblasts. Fibroblasts were grown to 70% confluence before undergoing growth arrest in serum-free medium for 48 h. Cells were then incubated in serum-free medium alone or serum-free medium containing 400 ng/ml BMP7 and were subsequently fixed and stained for EEA1 (green) and Hyal1 or Hyal2 (red) (A) and LAMP1 (green) and Hyal1 or Hyal2 (red) (B). Areas of co-localization appear as yellow, original magnification ×400. Co-localization scatter plots were performed to confirm co-localization (depicted as yellow areas), and Pearson's correlation was used to confirm statistical significance.