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. 2015 Mar 6;290(18):11235–11245. doi: 10.1074/jbc.M114.619718

FIGURE 2.

FIGURE 2.

His tag-labeled metal-chelate-affinity chromatography-purified ZmEXPB6 expressed in E. coli. Cell lysate, cells induced with IPTG; wash, third washing step shown; eluate, second elution step shown. Expansin identity in eluate was confirmed by WB (data not shown).