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. 2015 Mar 6;290(18):11235–11245. doi: 10.1074/jbc.M114.619718

FIGURE 6.

FIGURE 6.

Clustal O (1.2.1) multiple amino acid sequence alignment and fragmentation spectrum of ZmEXPB6 peptide. A, alignment between Z. mays (Zm) β-expansin (EXPB) 2 (AF332175), ZmEXPB3 (AF332176), ZmEXPB4 (AF332177), ZmEXPB5 (AF332178), ZmEXPB6 (AF332179), ZmEXPB7 (AF332180), and ZmEXPB8 (AF332181). ZmEXPB6 was identified by the underlined peptide sequence TGAGPLDNG by mass spectrometry analysis of proteins extracted from a PVDF blotting membrane. B, 19 fragmentation spectra of peptide 730.29 (2+) were recorded in positive ion modus with an ion score of 5.12E3, and spectra were combined for peptide sequence analysis.