FIGURE 5.

Determination of Avi-NaalL DPP IV activity. DPP IV activity was assayed using the fluorogenic substrate Gly-Pro-aminomethylcoumarin (excitation/emission, 335 nm/450 nm). Potential DPP IV activity of recombinant Avi-NaalL (1 μg) was assessed. Avi-GCPII (1 μg) and substrate alone (blank) were used as negative controls, whereas LNCaP lysate (10 μg of total protein) was used as a positive control. The reactions were incubated for 15 h at 37 °C. Each reaction was performed in triplicate and is shown as a mean with S.D. (error bar). RFU, relative fluorescence units.