FIGURE 8.

Avi-NaalL-catalyzed hydrolysis of two peptide substrates identified by PICS. Recombinant Avi-NaalL was mixed with peptide (peptide 52 or 71) in final concentrations of 1 and 100 μm, respectively. The specificity of cleavage was investigated using the catalytically ineffective mutant Avi-NaalL(E416A). Reaction mixtures were incubated for 15 h at 37 °C and analyzed using LC/MS. Peptide sequences or individual amino acids were identified based on their molecular mass and are shown in the key. The amounts of peptide or amino acid were determined using absorption at 210 nm. Each reaction was done in duplicate, and the result is shown as a mean with S.E (error bar).