FIGURE 7.

Selective insulin resistance in adipose tissue of ob/ob mice. A, intraperitoneal glucose tolerance tests were performed on 10-week-old ob/ob mice and age-matched wild-type controls at a dosage of 2g/kg of body weight. B, fasting insulin was assessed via ELISA following a 4-h fast. C, adipose tissue explants were stimulated with the indicated doses of insulin, and [³H]2-deoxyglucose (³H-DG) uptake was determined. The results are presented as a percentage of maximal insulin response. D, lipolysis was assessed in the absence and presence of insulin (100 nm) following stimulation with 0, 1, and 5 nm isoproterenol. B, basal; I, insulin. E, lipolysis data from D are presented as percentage inhibition by insulin to show the intact insulin response in ob/ob mice for all isoproterenol doses. F, adipose tissue lysates from explants experiments were immunoblotted using antibodies raised against the indicated proteins. G and H, lysates prepared from white adipose tissue collected from 4-h fasted WT and ob/ob mice were immunoblotted using antibodies raised against the indicated proteins. The data are means ± S.E., n = 3–6. *, p < 0.05 versus WT; **, p < 0.05 versus 0 insulin condition. Ins, insulin.