FIGURE 9.
Brpf1 regulates gene expression. A, RT-qPCR analysis in control and Brpf1-deficient embryos at E8.75. Three pairs of embryos were used. B, RT-qPCR analysis in Brpf1f/f and Brpf1−/− MEFs. The experiment was performed in triplicate with three batches of MEFs isolated from three independent litters as in Fig. 8, A–C. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not statistically significant. C, a simplified model illustrates how the Brpf1-Ing5-Eaf6 trimeric complex may recruit histone acetyltransferases (HAT) such as Moz/Morf and Hbo1 to regulate gene expression. In addition to multiple histone binding domains that Brpf1 possesses, Ing5 has its own PHD finger to recognize methylated histone H3. Brpf1 may also act through an unknown partner(s) as indicated by the red question mark. The black question mark denotes that the recruitment may lead to either transcriptional activation or repression. Ac, acetylation.