TABLE 1.

RT-qPCR primers and validation of selected genes

RT-qPCR results are shown as the mean ± S.D. ns, not statistically significant.

Target genes	Primer sequence		-Fold change		Primer position	GenBankTM accession no.
	5′	3′	E8.75 embryos	E15.5 MEFs
Brpf1-ex	CAGTAAGATCACCAACCGCC	GAGGAAAGGGGTCAGCTGCA	0.19 ± 0.05a	0.03 ± 0.01a	1711–2050	NM_030178.1
Brpf1-N	CAGCCCCTCTGAAGTCTCAC	CTAGTGCATTGGGGTCACCT	1.14 ± 0.26 ns	0.24 ± 0.05b	379–956	NM_030178.1
Brpf2	AACACTGACCTACGCACAAGC	GCCTCTCGCTGTTCTCCTTATT	0.84 ± 0.15 ns	1.24 ± 0.29 ns	87–247	NM_001033274
Brpf3	ACAAGCTCAAGATGCTAGAAGGC	TAGCTGGAAGTGACAAAGGCA	0.84 ± 0.22 ns	1.42 ± 0.27 ns	3485–3608	NM_001081315
Moz	ATGGTAAAACTCGCTAACCCG	CGTCCCGTCTTTGACGCTC	0.90 ± 0.10 ns	0.91 ± 0.16 ns	1–177	NM_001081149
Morf	AGAAGAAAAGGGGTCGTAAACG	GTGGGAATGCTTTCCTCAGAA	0.86 ± 0.09 ns	1.02 ± 0.11 ns	2474–2668	NM_017479
Hbo1	ATGCCGCGAAGGAAGAGAAAT	TCTTGGGAACTCTGGCTTAGC	0.99 ± 0.07 ns	1.16 ± 0.12 ns	1–164	NP_808287
Mof	CCGGATAGCACCTGGCATTC	CATACTTCACTTTGGTGATC	1.14 ± 0.12 ns	0.87 ± 0.53 ns	211–550	NM_026370
Hoxa9	CCCCGACTTCAGTCCTTGC	GATGCACGTAGGGGTGGTG	0.93 ± 0.06 ns	0.84 ± 0.26 ns	144–277	NM_010456
Rpls14	TGCCACATCTTTGCATCCTTC	ACTCATCTCGGTCAGCCTTCA	0.76 ± 0.07 ns	1.10 ± 0.04 ns	91–205	NM_020600
Rps19	CAGCAGGAGTTCGTCAGAGC	CACCCATTCGGGGACTTTCA	0.75 ± 0.17 ns		31–102	NM_023133
Rpl10l	ACCCAAAGTCCCGTTTCTGC	CTCGTCCGACACCATGTGG	0.21 ± 0.03c	1.03 ± 0.49 ns	50–168	NM_001162933
Rpl26	ACTTCTGACCGAAGCAAGAAC	CCGAATGGGCATAGACCGAA	0.72 ± 0.17 ns		22–150	NM_009080
Rpl41	CATCTTCCTTGAGACTCCTGC	CATCCCTCACTTCTGCTCC	0.78 ± 0.20 ns		16–167	NM_018860
Rps29	GTCTGATCCGCAAATACGGG	AGCCTATGTCCTTCGCGTACT	0.79 ± 0.20 ns		86–154	NM_009093
p16	GGGTTTCGCCCAACGCCCCGA	TGCAGCACCACCAGCGTGTCC	0.65 ± 0.55 ns	2.16 ± 0.14b	190	NM_001040654
p19	GTTTTCTTGGTGAAGTTCGTGC	TCATCACCTGGTCCAGGATTC	0.55 ± 0.57 ns	0.93 ± 0.15 ns	134	NM_009877
p15	CCCTGCCACCCTTACCAGA	CAGATACCTCGCAATGTCACG	1.36 ± 1.01 ns	1.45 ± 0.20 ns	204–372	NM_007670
p27	TGTCAGCGGGAGCCGCCAGG	ATATCTTCCTTGCTTCATAA		0.65 ± 0.08c	843–1182	NM_009875.4
p57	CCAAGCTGGACAGGACAAGC	AGTCCCAGCGGTTCTGGTCC		1.27 ± 0.41 ns	141–400	NM_001161624
p21	CACAGCGACCATGTCCAATC	GCGGGGCTCCCGTGGGCACT		1.26 ± 0.53 ns	201–680	U09507.1
Bax	TGAAGACAGGGGCCTTTTTG	AATTCGCCGGAGACACTCG		1.23 ± 0.04b	59–198	NM_007527
Cxcl15	CAAGGCTGGTCCATGCTCC	TGCTATCACTTCCTTTCTGTTGC	0.79 ± 0.03 ns	1.12 ± 0.28 ns	10–192	NM_011339
Fgf2	GCGACCCACACGTCAAACTA	TCCCTTGATAGACACAACTCCTC	0.89 ± 0.36 ns		164–225	NM_008006
Thpo	GGCCATGCTTCTTGCAGTG	AGTCGGCTGTGAAGGAGGT	0.73 ± 0.28 ns		27–140	NP_033405
Thsd7a	AGGTGCCCACCCTCTATCTG	TGTATGTAACGTAGTCCAGCCT	0.51 ± 0.22 ns		128–258	NM_001164805
Tailless	GGGAAGCACTACGGGGTCTA	GTGTCTTGTCTACGGGGCAT	0.33 ± 0.14 ns		73–196	NM_152229
Fbxo40	TGCCTCAACTCTGAGTATGGC	GAATGTTTCTGAGTCCACGTTTG	1.02 ± 0.24 ns		193–330	NM_001037321
unknown	AACCACCTGGGAAGACAGGA	TCCCTTCACACACACAGCCT	1.08 ± 0.65 ns		735–846	NR045476
Scp3l	AGTGCCTTTTGTCAGCAACAG	AGCGTGTCATTTGCCTGCTT	11.51 ± 6.38c	3.20 ± 0.71c	418–509	NM_001033423
Pax9	CGCACGCAGTGAATGGATTG	GCTGGTGTAGGGTAAGGAGC	0.97 ± 0.18 ns		716–845	NM_011041
Pcdhb22	TGTGATGGAGGAAACACCCAG	GCTTCCCAGTCTGTAGATCCAG	1.32 ± 0.02 ns		96–116	NM_053147
Fabp4	AAGGTGAAGAGCATCATAACCCT	TCACGCCTTTCATAACACATTCC	1.55 ± 0.70 ns		238–370	NM_024406
Tnfrsf13c	TCTGGTGAGAAACTGCGTGTC	GTCAGCGCCAGTATCAGTCC	1.19 ± 0.52 ns		90–260	NM_028075
Pou2af1	CACCAAGGCCATACCAGGG	GAAGCAGAAACCTCCATGTCA		0.92 ± 0.36 ns	44–224	NM_011136
Hemgn	GGAGGCAGACATCACAATGG	CCTTTTGCTCCACGTTCCCTT	1.45 ± 1.82 ns		146–256	NM_053149
Mecom	AAGTAATGAGTGTGCCTATGGC	AGTTGACTCTCGAAGCTCAAAC	0.73 ± 0.21 ns		36–264	NP_067417
CD31	ACGCTGGTGCTCTATGCAAG	TCAGTTGCTGCCCATTCATCA	1.12 ± 0.74 ns		22–130	NP_032842
Inpp4a	ACTCCATCGCTAGATCGAAAACC	AGGCAATGCTGCTTAGAAAGAT	0.83 ± 0.51 ns		169–307	NP_084542
Nek10	ATTACGTTCGTGATATTGGTGCT	TGACCACTACGCTTTCTAACCT	0.78 ± 0.26 ns		1418–1625	NM_001195229
Il22ra1	ATGAAGACACTACTGACCATCCT	CAGCCACTTTCTCTCTCCGT	0.62 ± 0.43 ns		1–198	NM_178257
Gapdh	TGATGACATCAAGAAGGTGGTGAA	TCTTACTCCTTGGAGGCCATGT	as reference		814–1059	XM_001476707.3

^a p < 0.001.

^b p < 0.01.

^c p < 0.05.