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Free thiol content of Cd-MsrA upon l-Met-SO reduction, as determined by reaction with DTNB
Stopped flow was used to determine the thiol content of WT, C206S, C215S, and C87S/C206S mutants in the presence and absence of l-Met-SO.
| Without l-Met-SO |
With l-Met-SO |
|||
|---|---|---|---|---|
| Expected value | Obtained value | Expected value | Obtained value | |
| WT | 4 | 3.5 | 1 | 0.6 |
| C206S | 3 | 2.8 | 1 | 1.2 |
| C215S | 3 | 2.8 | 1 | 1.1 |
| C87S/C206S | 2 | 1.7 | 0 | 0.3 |
