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. 2015 Mar 9;290(18):11365–11375. doi: 10.1074/jbc.M114.632596

TABLE 1.

Free thiol content of Cd-MsrA upon l-Met-SO reduction, as determined by reaction with DTNB

Stopped flow was used to determine the thiol content of WT, C206S, C215S, and C87S/C206S mutants in the presence and absence of l-Met-SO.

Without l-Met-SO
With l-Met-SO
Expected value Obtained value Expected value Obtained value
WT 4 3.5 1 0.6
C206S 3 2.8 1 1.2
C215S 3 2.8 1 1.1
C87S/C206S 2 1.7 0 0.3