FIGURE 2.

The C-terminal wrapping loop regulates access to binding modes. a, EMSA as a function of -fold excess of the different DBD constructs for 30 nm TeloA, labeled at the 5′-end of the top strand with FAM (see “Experimental Procedures”). b, same experiments as in a but for HMRE. c and d, EMSA as a function of -fold excess of the different DBD constructs targeting the protein-DNA complexes formed with 2 μm unlabeled TeloA in c and HMRE in d. e, change in fluorescence anisotropy of dsDNAs FAM-labeled at the 5′-end of the top strand. The experiments were performed with 255 nm TeloA (black circles), RND (gray circles), or HMRE (open circles). Shown are the data for DBD⁶¹⁵ (left), DBD⁵⁹⁰ (middle), and DBD⁵⁷⁹ (right).