FIGURE 3.

DBD⁸²⁷ can access higher stoichiometry on DNA and it is regulated by the latch and tox regions. a, EMSA targeting the protein-DNA complexes as a function of -fold excess of the DBD⁸²⁷, DBD^827/ΔLatch, and DBD^827/ΔTox for 2 μm unlabeled TeloA, RPG, HMRE, and TeloS. b, change in relative total fluorescence intensity as a function of DBD⁸²⁷/DNA ratio for 255 nm TeloA (gray) or HMRE (black) FAM-labeled at the 3′-end. Inset, same data for the DBD⁶¹⁵ construct. c, change in relative total fluorescence intensity for 255 nm HMRE (FAM-labeled at the 3′-end) for the three different DBD⁸²⁷ constructs.