FIGURE 6.

Hepatic Sort1 overexpression prevented the triglyceride-raising effect of PX866 in wild type mice. Male 12-week-old C57BL6J mice were intravenously injected with Ad-GFP or Ad-Sort1 at 5 × 10⁸ pfu/mouse. After 5 days, mice received either vehicle (veh) or 8 mg/kg PX866 intraperitoneal injection once daily for an additional 3 days. Mice were sacrificed after an 8-h fast. A, liver Sort1 protein was measured by immunoblot. B, H&E staining of liver sections. C and D, plasma triglyceride (C) and cholesterol (D) were measured with assay kits. E, liver mRNA expression was measured by real-time PCR. Results in C–E are plotted as mean ± S.E. (error bars) (n = 4). p ≤ 0.05 is considered as statistical significance; *, versus Ad-GFP + vehicle group; **, versus Ad-Sort1 + vehicle group; #, versus Ad-GFP + PX866 group.