FIGURE 3.

Thermodynamics of zinc-Hint binding. A, schematic diagram of the shortened precursor construct used for producing Hint sample in ITC and NMR studies. It contains a His tag and four native residues (HVHG) from HhN C terminus followed by Drosophila melanogaster Hint domain. The Hint domain is obtained after DTT induced cleavage from the precursor. B, ITC data for the titration of Zn²⁺ into Hint at 25 °C. The upper portion contains the baseline-corrected raw data, and the lower portion indicates the concentration normalized heat from titration at the molar ratio of Hint.