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NTMT1 methylated the RCC1-10 and Me-RCC1-10 in a distributive fashion. A, population distribution of RCC1-10 methylated states over time. B, population distribution of MeRCC1-10 methylated states over time. Unmethylated, monomethylated, dimethylated, and trimethylated RCC1-10 were quantified by mass spectrometry. The reactions were carried out with 0.2 μm NTMT1, 10 μm RCC1-10 or Me-RCC1-10, and 50 μm AdoMet in 25 mm Tris, 50 mm KCl, and 2 mm DTT. Error bars, S.D.
