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. 2015 Mar 18;290(18):11611–11622. doi: 10.1074/jbc.M115.642017

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Assay for the assembly of Hsp60 complexes. A, ³⁵S-labeled Hsp60 was imported into isolated WT mitochondria at the indicated temperatures and time points. Proteins were analyzed either by SDS-PAGE (SDS) or by blue native (BN) electrophoresis and autoradiography. B, ³⁵S-labeled Hsp60 was imported into isolated WT mitochondria. Mitochondria were lysed with the indicated detergents (Deterg.) and subjected to blue native electrophoresis. Protein complexes were detected by autoradiography. Dig., digitonin; TX-100, Triton X-100.