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. 2015 Mar 21;290(18):11623–11634. doi: 10.1074/jbc.M115.642256

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Light-induced difference spectra of two chimeric channelrhodopsins (ChR_5/2 and ChR_2/5) containing mutations at the DC gate (Asp-195 and Cys-167) in the frequency region of the C=O stretching vibration of carboxyl group (1800–1670 cm⁻¹). A, ChR_5/2 (green) and its D195N mutant (blue). B, ChR_2/5 (magenta) and its D195N mutant (blue). C, ChR_5/2 (green) and its C167S mutant (red). D, ChR_2/5 (magenta) and its C167S mutant (red).