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Light-induced difference spectra of the D195N mutants of two chimeric channelrhodopsins compared with that of the corresponding D156N mutant of ChR2. A, ChR5/2. B, ChR2/5. The difference spectra of the Asp mutants of ChR5/2, ChR2/5, and ChR2 are colored green, magenta, and orange, respectively. The double difference spectra were calculated by subtracting the light-induced difference spectrum of ChR2 with that of each chimera.
